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EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 250 preps

EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 250 preps

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  • Brand: Blirt
  • Catalog No.: EM26.1-250
  • Quantity/Unit: 250 preps/pack
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Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 250 preps supplied by BLIRT is intended to ensure a quick and effective DNA purification after enzymatic reactions and and directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer).. It effectively eliminates nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, agarose, ethidium bromide and other contaminants, etc. The purified DNA can be utilized in general downstream applications. The protocol of isolation and buffer formulations was optimized to ensure high efficiency of isolation and purity of pDNA. The process for DNA purification uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st isolation step involves CB Buffer is added to DNA sample. This leads to the degradation of proteins and enabling DNA to bind with the columns membrane while in the gel-out protocol DNA fragments is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. The binding buffer has a color indicator that allows an easy monitoring of the solutions pH for optimal DNA binding. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications such as PCR, qPCR, DNA sequencing, enzymatic restriction, DNA ligation, etc. or stored until ready to use.

Product Specifications:

Product name - EXTRACTME DNA CLEAN-UP & GEL-OUT KIT, 250 preps

Catalog No - EM26.1-250

Brand: BLIRT

Pack size: 250 preps

Product Features:

1 SAMPLE MATERIAL

CLEAN-UP: up to 200 l of a DNA sample

GEL-OUT: agarose fragment of up to 300 mg containing DNA.

2. YIELD- Depending on DNA fragment length (in the range of 100 bp 10 kb):

CLEAN-UP: 60-99%

GEL-OUT: 7095%

3. BINDING CAPACITY

approx. 40 g DNA

4. DNA FRAGMENT LENGTH

100 bp 10 kb

DNA fragments in the range of 50100 bp and 1020 kb can also be purified as can genomic and plasmid DNA, however the efficiency will be decreased.

5. TIME REQUIRED

510 min for clean-up procedure

1620 min for gel-out procedure

6. DNA PURITY

A260/A280 ratio = 1.7 1.9

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