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EXTRACTME DNA CLEAN-UP KIT, 50 preps

EXTRACTME DNA CLEAN-UP KIT, 50 preps

₹ 4,551.75 *
(0)
  • Brand: Blirt
  • Catalog No.: EM07.1-050
  • Quantity/Unit: 50 preps/pack
  • Shipping timeline will be mentioned in quote

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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EXTRACTME DNA CLEAN-UP KIT, 50 preps supplied by BLIRT is intended to ensure a quick and effective DNA purification after enzymatic reactions. It effectively eliminates nucleases, primers, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, salts, mineral oil, etc. The purified DNA can be utilized in general downstream applications. Primers from PCR reactions are eliminated quantitatively while small DNA fragments are still bound and purified with high recovery. The protocol of isolation and buffer formulations was optimized to ensure high efficiency of isolation and purity of pDNA. The process for DNA purification uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st isolation step involves CB Buffer is added to DNA sample. This leads to the degradation of proteins and enabling DNA to bind with the columns membrane. The binding buffer has a color indicator, that allows an easy monitoring of the solutions pH for optimal DNA binding. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications such as PCR, qPCR, DNA sequencing, enzymatic restriction, DNA ligation, etc. or stored until ready to use.

Product Specifications:

Product name - EXTRACTME DNA CLEAN-UP KIT, 50 preps

Catalog No - EM07.1-050

Brand: BLIRT

Pack size: 50 preps

Product Features:

1 SAMPLE MATERIAL

up to 200 l of DNA sample.

2. YIELD

60-99% depending on DNA fragment length

3. BINDING CAPACITY

approx. 40 g DNA

4. DNA FRAGMENT LENGTH

50 bp ~20 kbp

genomic and plasmid DNA, however the efficiency will be decreased

5. TIME REQUIRED

10 min for 6 PCR purifications

6. DNA PURITY

A260/A280 ratio = 1.7 1.9

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