EXTRACTME DNA SWAB & SEMEN KIT, 50 preps
- Brand: Blirt
- Catalog No.: EM06-050
- Quantity/Unit: 50 preps/pack
EXTRACTME DNA SWAB & SEMEN KIT, 50 preps supplied by BLIRT is intended for a fast and effective purification of of high quality DNA from human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs) as well as from semen. The process for DNA purification involves 4 steps and uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st step involves the swab or semen sample. DTT must also be used while isolating from semen. All the cellular membranes and proteins gets degraded in this stage. The lysate is applied to the purification minicolumns membrane post the addition of chaotropic salts and the DNA is bound. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.
Product Specifications:
Product name - EXTRACTME DNA SWAB & SEMEN KIT, 50 preps
Catalog No - EM06-050
Brand: BLIRT
Pack size: 50 preps
Product Features:
1 SAMPLE MATERIAL
Buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen.
2. BINDING CAPACITY
Approx. 25 g DNA
3. TIME REQUIRED
45-50 minutes (including incubation time)
4. DNA PURITY
A260/A280 ratio = 1.7 1.9