EXTRACTME DNA TISSUE KIT, 250 preps
- Brand: Blirt
- Catalog No.: EM03-250
- Quantity/Unit: 250 preps/pack
EXTRACTME DNA TISSUE KIT, 250 preps supplied by BLIRT is intended for a fast and effective purification of of high quality DNA from solid tissues (fresh, frozen, formalin-preserved or paraffin-embedded), physiological fluids, hair, rodent tails, insects and cell cultures. The protocol for isolation and buffer formulations is optimized for high isolation efficiency and DNA purity. The process for DNA purification involves 4 steps and uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st step includes the lysing of biological material by Proteinase K in optimized GL Buffer. All the cellular membranes and proteins gets degraded in this stage. The utilization of RNase A is advised only when it is necessary to eliminate RNA. The lysate is applied to the purification minicolumns membrane post the addition of chaotropic salts and the DNA is bound. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications like PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.
Product Specifications:
Product name - EXTRACTME DNA TISSUE KIT, 250 preps
Catalog No - EM03-250
Brand: BLIRT
Pack size: 250 preps
Product Features:
1.SAMPLE MATERIAL
fresh or frozen solid tissue: 130 mg
formalin-preserved tissue: 130 mg
paraffin-embedded tissue: 130 mg
cell culture: 103107 cells
physiological fluids (urine, PMR, peritoneal fluid): 15 ml
hair: 1030 mg
insects: 130 mg
rodent tail: up to 30 mg
2. BINDING CAPACITY
Approx. 50 g DNA
3. TIME REQUIRED
approx. 12 minutes (lysis time not included)
3040 minutes for mechanical homogenization
116 hours for periodical shaking by vortexing
4. DNA PURITY
A260/A280 ratio = 1.7 1.9