WORLDWIDE SHIPPING
EXTRACTME DNA TISSUE KIT, 250 preps

EXTRACTME DNA TISSUE KIT, 250 preps

(0)
  • Brand: Blirt
  • Catalog No.: EM03-250
  • Quantity/Unit: 250 preps/pack

Share:

EXTRACTME DNA TISSUE KIT, 250 preps supplied by BLIRT is intended for a fast and effective purification of of high quality DNA from solid tissues (fresh, frozen, formalin-preserved or paraffin-embedded), physiological fluids, hair, rodent tails, insects and cell cultures. The protocol for isolation and buffer formulations is optimized for high isolation efficiency and DNA purity. The process for DNA purification involves 4 steps and uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st step includes the lysing of biological material by Proteinase K in optimized GL Buffer. All the cellular membranes and proteins gets degraded in this stage. The utilization of RNase A is advised only when it is necessary to eliminate RNA. The lysate is applied to the purification minicolumns membrane post the addition of chaotropic salts and the DNA is bound. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications like PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

Product Specifications:

Product name - EXTRACTME DNA TISSUE KIT, 250 preps

Catalog No - EM03-250

Brand: BLIRT

Pack size: 250 preps

Product Features:

1.SAMPLE MATERIAL

fresh or frozen solid tissue: 130 mg

formalin-preserved tissue: 130 mg

paraffin-embedded tissue: 130 mg

cell culture: 103107 cells

physiological fluids (urine, PMR, peritoneal fluid): 15 ml

hair: 1030 mg

insects: 130 mg

rodent tail: up to 30 mg

2. BINDING CAPACITY

Approx. 50 g DNA

3. TIME REQUIRED

approx. 12 minutes (lysis time not included)

3040 minutes for mechanical homogenization

116 hours for periodical shaking by vortexing

4. DNA PURITY

A260/A280 ratio = 1.7 1.9

Related Products