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EXTRACTME PLASMID MINI DNA KIT, 50 preps

EXTRACTME PLASMID MINI DNA KIT, 50 preps

₹ 4,819.50 *
(0)
  • Brand: Blirt
  • Catalog No.: EM01.1-050
  • Quantity/Unit: 50 preps/pack
  • Shipping timeline will be mentioned in quote

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Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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EXTRACTME PLASMID MINI DNA KIT, 50 preps supplied by BLIRT is intended for a fast and effective purification of high quality plasmid DNA from recombinant Escherichia coli strains. The protocol for isolation and buffer formulations was optimized to get higher isolation efficiency and DNA purity. The process for DNA purification involves 4 steps and uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively. The 1st step involves the release of plasmid DNA from bacterial cells by alkaline lysis. This is followed by the neutralization of lysate and separation of all cell residues along with proteins and genomic DNA by centrifugation. Lysate is applied to purification minicolumn membrane and DNA is bound. The two-step washing stage adequately eliminates pollutants and enzyme inhibitors. The elution of purified DNA is done with the help of either a low ionic strength buffer (Elution Buffer) or water (pH 7.09.0). And it may be utilized directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

Product Specifications:

Product name - EXTRACTME PLASMID MINI DNA KIT, 50 preps

Catalog No - EM01.1-050

Brand: BLIRT

Pack size: 50 preps

Product Features:

1 SAMPLE MATERIAL

Bacterial broth culture, frozen cell pellet however the efficiency will be decreased.

2. BINDING CAPACITY

Approx. 60 g DNA

3. TIME REQUIRED

25 minutes

4. DNA PURITY

A260/A280 ratio = 1.7 1.9

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