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EXTRACTME TOTAL RNA MICRO SPIN KIT, 250 preps

EXTRACTME TOTAL RNA MICRO SPIN KIT, 250 preps

₹ 56,049.00 *
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  • Brand: Blirt
  • Catalog No.: EM31.1-250
  • Quantity/Unit: 250 preps/pack
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Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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EXTRACTME TOTAL RNA MICRO SPIN KIT, 250 preps supplied by BLIRT is intended to ensure a quick and effective purification of high quality RNA up to 15 mg of tissue (fresh or frozen) and up to 106 cultured cells with an extremely low elution volume of only 5 l. The protocol of isolation and buffer formulations was optimized to ensure high efficiency of isolation and purity of RNA. The process for RNA purification uses spin minicolumns with membranes which bind nucleic acids efficiently and selectively at high concentration of chaotropic salts. The 1st isolation step involves the homogenization of a tissue which results in the disintegration of intercellular bonds (epithelial tissue) and fragmentation of high-molecular proteins (muscle or connective tissue). Lysing of the homogenate is done with guanidine thiocyanate and detergents. Guanidine thiocyanate and -mercaptoethanol (optional) is utilized to inactivate RNases. The separation of the homogenate from undigested tissue/cell that remains after centrifugation is done. The RNA then binds to the RNA Purification Column membrane by selective conditions in mixture after adding ethanol. A three-step washing stage adequately eliminates contaminations and protein inhibitors. By the help of low ionic strength buffer or RNasefree water (pH 7.0-9.0) the purified RNA is eluted out that may be utilized in several downstream applications, like PCR, qPCR, primer extension, sequencing, microarrays, Southern blotting, and in case of RNA also Northern blotting, RT-PCR, cDNA synthesis or stored until ready to use.

Product Specifications:

Product name - EXTRACTME TOTAL RNA MICRO SPIN KIT, 250 preps

Catalog No - EM31.1-250

Brand: BLIRT

Pack size: 250 preps

Product Features:

1 SAMPLE MATERIAL

fresh or frozen tissue (stored at -80C): up to 15 mg

tissue preserved in RNase inactivating buffers: up to 15 mg

cell culture: up to 106 cells

2. BINDING CAPACITY

Approx. 30 g RNA

3. TIME REQUIRED

16-20 minutes (lysis and homogenization time not included)

30-60 minutes for homogenization in liquid nitrogen

30-40 minutes for mechanical homogenization (ceramic beads)

15 minutes for optional DNase I treatment

4. RNA PURITY

A260/A280 ratio = 1.7 1.9

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