Fischer's Medium With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.5 -7.1 whereas the pH with Sodium Bicarbonate is between 7.4 -8.0. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (91.00)

Magnesium chloride anhydrous (46.83)

Potassium chloride (400.00)

Sodium chloride (8000.00)

Sodium dihydrogen phosphate anhydrous (60.00)

Sodium phosphate dibasic anhydrous (60.00)

Amino acids

Choline chloride (1.50)

D-Biotin (0.01)

D-Ca-Pantothenate (0.50)

Folic acid (10.00)

Niacinamide (0.50)

Pyridoxal hydrochloride (0.50)

Riboflavin (0.50)

Thiamine hydrochloride (1.00)

myo-Inositol (1.50)

D-Glucose (1000.00)

Phenol red sodium salt (5.30)

Fischer's medium was originally formulated to support serial propagation of cells from leukemic mice. Fischer's medium supports clonal reproduction of cells, particularly lymphoblast from primary explants or from cells in culture. This product contains L-glutamine. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.