Glasgow's Minimum Essential Medium (GMEM) With L-Glutamine and Tryptose Phosphate Broth Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.7 -6.3 whereas the pH with Sodium Bicarbonate is between 7.1 -7.7. The osmolality without Sodium Bicarbonate is in the range of 235.00 -275.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 295.00 -335.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonhydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (2.00)

D-Ca-Pantothenate (2.00)

Folic acid (2.00)

Nicotinamide (2.00)

Pyridoxal hydrochloride (2.00)

Riboflavin (0.20)

Thiamine hydrochloride (2.00)

i-Inositol (3.60)

D-Glucose (4500.00)

Phenol red Sodium Salt (15.00)

Tryptose Phosphate Broth (2950.00)

Glasgow's Minimum Essential Medium is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating the genetic factors affecting cell competence. This product contains L-glutamine and tryptose phosphate broth. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.