Specific antibodies are used commonly for the identification and characterization of proteins during techniques such as ELISA, immune-electrophoresis, western blotting etc. These antibodies can be monoclonal or polyclonal, based on the requirement of the assay. Antibodies against a particular protein can be raised in various species of animals (hosts). The host animals are immunized with the protein and the antibodies generated in the host serum against the protein are collected and purified for use. The antibodies can also be conjugated to several labels, which can be used to detect the antibodies bound to specific target antigens for quantitative and qualitative analysis. The Goat anti mouse IgG FITC is a conjugated secondary antibody from Genei. This antibody is generated using goat as a host. IgG is procured from mouse serum and is injected into goat, as an immunogen. The antibody generated in the goat is isolated and purified via affinity chromatography. To enable its use as a secondary antibody, this product is conjugated to FITC (Fluorescein isothiocyanate). FITC is a fluorescent dye which has an absorption and emission wavelength of 450-495 nm. The amount of the bound secondary antibody can be quantified by measuring the FITC fluorescence.
Product name: Goat anti mouse IgG FITC
Quantity: 1 mL
Catalogue number: 1120000000000