Specific antibodies are used commonly for the identification and characterization of proteins during techniques such as ELISA, immune-electrophoresis, western blotting etc. These antibodies can be monoclonal or polyclonal, based on the requirement of the assay. Antibodies against a particular protein can be raised in various species of animals (hosts). The host animals are immunized with the protein and the antibodies generated in the host serum against the protein are collected and purified for use. The antibodies can also be conjugated to several labels, which can be used to detect the antibodies bound to specific target antigens for quantitative and qualitative analysis. The Goat anti Rabbit IgG HRP, adsorbed with bovine, human and mouse serum proteins is a conjugated secondary antibody from Genei. This antibody is generated using goat as a host. IgG is procured from rabbit serum and is injected into goat, as an immunogen. The antibody generated in the goat is isolated and purified via affinity chromatography. Immobilized serum proteins from bovine, human and mouse sources are used for adsorbing the antibodies. To enable its use as a secondary antibody, this product is conjugated to HRP (horse-radish peroxidase) via the periodate method. This enzyme uses hydrogen peroxidase to catalyse the oxidation of several substances, such as TMB. To quantify the amount of bound HRP conjugated antibody, the substrate must be added along with hydrogen peroxide. The resulting coloured product can be quantified using spectrometric methods.

Product specification:

Product name: Goat anti Rabbit IgG HRP, adsorbed with bovine, human and mouse serum Quantity: 0.5 mL

Brand: Genei

Catalogue number: 1142075001730

Storage: 4C