HEPES free base (Molecular grade)
Catalog No.: 219482725
CAS No: 7365-45-9
Quantity/Unit: 25 g/Bottle
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- PRODUCT DETAILS
HEPES by MP Biomedicals is a high grade reagent. Also known as 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, this is a zwitterionic buffer. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. The recommended pH range for this buffer is 6.8 8.2. CAS Number: 7365-45-9 Quantity provided: 25 gm per bottle Chemical formula: C8H18N2O4S Molecular weight: 238.30 HEPES has a wide range of applications in biological and chemical industries. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. It lacks the ability to form a significant complexes with most metal ions, hence is recommended for use as a non-coordinating buffer in solutions with metal ions. It is suitable for use in the bicinchoninic acid (BCA) assay. These reagents are suitable for industrial and research applications.