Hi-Zinc Stain is supplied by HiMedia. It is available in the form of a colourless solution. Hi-Zinc Stain is a zinc-based reversible, highly sensitive protein gel stain that rapidly develops protein bands on polyacrylamide gels after electrophoresis. This staining solution is compatible with both native and SDS-denatured gels and no de-staining is required for the visualization of protein bands. It is advisable to store this product between 15-25C. Hi-Zinc Stain contains two solutions:

10X Staining Solution I, ML057A (10X Imidazole Solution)

10X Staining Solution II, ML057B (10X Zinc sulphate Solution).

Hi-Zinc Stain is a unique staining solution as it functions in a reversible way as the protein bands on the polyacrylamide gels disappear completely after treatment with the Hi-Zinc Destain solution. This staining solution acts in a single step and is based upon the interaction of zinc ions with the protein bands and polyacrylamide gels. Hi-Zinc Stain turns the gel opaque white by depositing zinc metal precipitate in it while the SDS coating on the proteins prevents the stain from binding to the proteins and as a result a negative image of the gel is produced where clear protein bands are visualized against the opaque white polyacrylamide background. The simple two-step staining procedure is very rapid and sensitive compared to the standard Coomassie staining procedure. Gels stained with the Hi-Zinc Stain can be transferred for immunoblotting procedures after de-staining in 5 minutes with Hi-Zinc Destain. The solutions are supplied as 10X concentrate and should be diluted to 1X before use.