To be used in conjunction with Caymans HIF-1 Polyclonal Antibody (Item No. 10006421) to block protein-antibody complex formation during immunochemical analysis of HIF-1. HIF-1 is a transcription factor that accumulates under low-oxygen conditions.1,2 Following hypoxic stimulus and cytoplasmic accumulation, HIF-1 migrates to the nucleus where, with other transcription factors, it drives the production of stress-adaptive proteins. This response is essential for maintenance of normal oxidative physiology, however HIF-1 overexpression in cancer cells promotes tumor survival.2,3,4,5,6 HIF-1 is an EPAS-1 family member with phosphorylation dependent activities. HIF-1 will migrate to the apparent molecular weights of 110-125 kDa (by SDS-PAGE) depending on phosphorylation status.7,8