DMEM/F12, 1:1 Mixture w/ L-Alanyl-L-Glutamine, NaHCO3 w/o HEPES, Trace elements

DMEM/F12, 1:1 Mixture w/ L-Alanyl-L-Glutamine, NaHCO3 w/o HEPES, Trace elements

₹ 2,732.00


Catalog No.: AL155G-5X100ML

Quantity/Unit: 5x100ml/EA

Usually Shipped in: 1-2 Weeks

Min Orderable Qty : 1 Pack
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HiGlutaXL™ Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) With L-Alanyl-L-Glutamine and 2.438gms per litre Sodium bicarbonate Without HEPES buffer and Trace elements is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C and away from bright light. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (154.50) 

• Copper sulphate pentahydrate (0.001) 

• Disodium hydrogen phosphate (71.02)

• Ferric nitrate nonahydrate (0.05) 

• Ferrous sulphate heptahydrate (0.42) 

• Magnesium chloride hexahydrate (61.20) 

• Magnesium sulphate anhydrous (48.84) 

• Manganese sulphate (0.0001) 

• Potassium chloride (311.80)

• Sodium bicarbonate (2438.00)

• Sodium chloride (6996.00)

• Sodium dihydrogen phosphate (54.30) 

• Zinc sulphate heptahydrate (0.43)

• Amino acids

• Choline chloride (8.98)

• D-Biotin (0.003)

• D-Ca-Pantothenate (2.24)

• Folic acid (2.66) 

• Niacinamide (2.02)

• Pyridoxal hydrochloride (2.00) 

• Pyridoxine hydrochloride (0.03)

• Riboflavin (0.22)

• Thiamine hydrochloride (2.17) 

• Vitamin B12 (0.68)

• myo-Inositol (12.60)

• D-Glucose (3151.00) 

• DL-Thioctic acid (0.10) 

• Hypoxanthine (2.40) 

• Linoleic acid (0.04)

• Phenol red sodium salt (8.63)

• Putrescine hydrochloride (0.08) 

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL™ medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4ºC for longer periods. This is a 1X Liquid Cell Culture Medium with L-alanyl-L-glutamine and 2.438gms per litre sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.



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