HTC002-5PR is referred as HiPer Affinity Chromatography Teaching Kit under HiMedia catalogue. The kit is used to learn the technique of protein purification of Horse Radish Peroxidase (HRP) by affinity chromatography using Concanavalin A agarose and estimation of the activity as well as the protein concentration of the purified

protein.HTC002-5PR kit enables the purification of a protein on the basis of its reversible interaction with a ligand coupled to a chromatographic matrix. The kit includes Concanavalin A- Agarose Suspension, Standard Protein, Crude Protein Sample, Elution Buffer, Sodium acetate Buffer, H2O2, ABTS, Folins Reagent, Column.

Specifications:

No of Experiments performed -5

Duration of Experiment -3 hrs.

Pack Size 5PR/pack

Storage Conditions:

Kit: - Stable for 6 months from the date of manufacturing

Standard Protein: -20C

Concanavalin A-Agarose Suspension, Crude Protein Sample,

Elution Buffer, Sodium Acetate Buffer, Hydrogen Peroxide and ABTS: 2-8C

Other Kit contents: Room Temperature(15 to 25C)