HiPurA 96 Blood Genomic DNA Purification Kit is supplied by HiMedia. HiPurA 96 Blood Genomic DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, library screening and sequencing. The DNA purification procedure using the 96-well format comprises of three steps viz. adsorption of DNA to the silica gel membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias 96-well format allows rapid processing of multiple samples. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing. Kit contains:

Resuspension Solution (1X PBS)

Lysis Solution (C1)

Prewash Solution Concentrate (PW)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiPurA 96-well Block (1.6 ml)

HiPurA 96-well DNA Plate

HiPurA 96-well V-Block

HiPurA Silicon Pad for sealing

This kit simplifies isolation of DNA from fresh and old (more than 24 hours) blood. Genomic DNA purification from blood involves cell lysis, which is achieved by incubation of whole blood in a solution containing chaotropic ions in the presence of Proteinase K at 70C. HiMedias HiPurA 96-well DNA Plate contains specially developed membranes for optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit. Typical yield is 4-12 g of total DNA from 200 l of whole blood.