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HiPurA 96 Plasmid DNA Purification Kit-MB518-1X96PR

HiPurA 96 Plasmid DNA Purification Kit-MB518-1X96PR

₹ 13,230.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MB518-1X96PR
  • Quantity/Unit: 1X96pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HiPurA 96 Plasmid DNA Purification Kit is supplied by HiMedia. The HiPurA 96 Plasmid DNA Purification Kit provides a simple and rapid method for purifying plasmid or cosmid DNA from small volume of bacterial cultures (2.0 ml). This kit is not recommended for the purification of large recombinant constructs such as BACs and PACs. The kit uses the principle of silica binding in a 96-well format that eliminates the need for expensive resins, alcohol precipitation and hazardous organic compounds such as phenol and chloroform. This procedure is based on a modified alkaline lysis procedure specialized for high-throughput DNA applications. This kit contains:

Resuspension Solution (HP1)

Lysis Solution (HP2)

Neutralization Solution (HN3)

Wash Solution (HPB)

Wash Solution Concentrate (HPE)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

RNase A Solution (20 mg/ml)

HiPurA 96-well DNA Plate

HiPurA 96-well Block (2.2 ml)

HiPurA 96-well Block (1.6 ml)

HiPurA Silicon Pad for sealing

HiPurA Breathable Film

HiPurA 96-well V-Block

The harvested bacterial culture is lysed and collected by centrifugation after which it is applied on to the silica column for binding of the DNA molecules in the presence of high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in Elution Buffer. The purified plasmid DNA can be used for immediate use in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing.

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