HiPurA Bacterial Genomic DNA Purification Kit-MB505-50PR

HiPurA Bacterial Genomic DNA Purification Kit-MB505-50PR

₹ 7,880.00 *

Brand: HIMEDIA

Catalog No.: MB505-50PR

Quantity/Unit: 50pr*/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

HiPurA Bacterial Genomic DNA Purification Kit is supplied by HiMedia. HiPurA Genomic DNA Purification Kits provide a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting. This kit contains:

Gram Positive Lysis Solution (GPLA)

Lysis Solution (AL)

Lysis Solution (C1)

Prewash Solution (PWB)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

Lysozyme

HiElute Miniprep Spin Column (Capped) [in DBCA16 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from bacteria (Gram positive and Gram negative) by the spin-column procedure. Bacterial cells are grown in a medium till they reach log phase and are harvested by centrifugation. After harvesting, the bacterial (Gram positive) cell wall is degraded by lysozyme and Proteinase K. For Gram negative bacteria, the lysozyme treatment is not required. Following lysis, the DNA is bound to the silica-gel membrane of the HiElute Miniprep Spin Column (Capped) to yield approximately upto 20g of pure DNA. Two rapid wash steps remove trace amount of salt and protein contaminants resulting in the elution of pure DNA in the Elution Buffer provided with the kit.