MBT143-10R is referred as HiPurA BL21(DE3) Competent Cells under HiMedia catalogue. HiPurA BL21(DE3) Competent Cells are specially designed chemically competent BL21(DE3) cells to make cloning experiments more easier.
Competent cells are ready to use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA BL21(DE3) Competent Cells prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for regular sub cloning of recombinant plasmids.
Genotype: - F ompT hsdSB (rB, mB) gal dcm (DE3)
Applications:1)Highest transformation efficiency 2) General cloning 3) Blue-white selection 4) Plasmid isolation
Pack size 20 vials