HiPurA Bone DNA Purification Kit-MB525-10PR
₹ 4,830.00 *
Catalog No.: MB525-10PR
Usually Shipped in: 2-3 Weeks
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HiPurA Bone DNA Extraction Kit is supplied by HiMedia. HiPurA Bone DNA Extraction Kit provides a fast and easy method for isolation of genomic DNA from bone samples of various species (including forensic samples). The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. This kit contains:
Bone Extraction Buffer
Binding Buffer (BBH)
Glass Powder Suspension (GPS)
Wash Solution Concentrate (BW)
Elution Buffer (ET) [10mM Tris-Cl, pH 8.5]
This kit simplifies the isolation of DNA from bone. Once the bone sample is decalcified, it is subjected to lysis by Bone Extraction Buffer. Following the lysis step is the binding of DNA. Two rapid wash steps remove trace salt and protein contaminants resulting in the elution of high quality DNA in the Elution Buffer provided with the kit. The yield of bone DNA depends on the sample type. A single elution with 100l of Elution Buffer will provide sufficient DNA to carry out multiple amplification reactions. The eluted DNA is suitable for direct use in PCR, restriction digestion, and Southern blotting applications. It is advisable to store this product between 15-25C.