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HiPurA Mammalian Genomic DNA Purification Kit-MB506-50PR

HiPurA Mammalian Genomic DNA Purification Kit-MB506-50PR

₹ 6,040.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MB506-50PR
  • Quantity/Unit: 50pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HiPurA Mammalian Genomic DNA Purification Kit is supplied by HiMedia. HiPurA Mammalian Genomic DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR and Southern blotting technique etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality genomic DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. This kit contains:

Resuspension Solution (1X PBS)

Lysis Solution (C1)

Lysis Solution (AL)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from tissues or cells with spin-column procedure. Animal tissue (spliced and digested), suspended/attached animal cells, whole blood or digested rodent tail tissue is subjected to lysis by Proteinase K in a chaotropic salt solution. Following lysis is the binding of DNA to the silica gel membrane of the HiElute Miniprep Spin Column (Capped) to yield high purity DNA. Two rapid wash steps removes trace salt and protein contaminants resulting in the elution of high quality DNA in the Elution Buffer (ET) provided with the kit. Typical DNA yield from 1 x 106 cells is 10-20 g and 30 mg of tissue yields 30-45 g of DNA.

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