HiPurA Plasmid DNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Plasmid DNA Miniprep Purification Kit provides a fast and easy method for purification of plasmid DNA for reliable applications in PCR, library screening, sequencing, etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure plasmid DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing. This kit contains:

Resuspension Solution (HP1)

Lysis Solution (HP2)

Neutralization Solution (HN3)

Wash Solution (HPB)

Wash Solution Concentrate (HPE)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

The harvested bacterial culture is lysed and collected by centrifugation after which it is applied on to the silica column for binding of the DNA molecules in the presence of high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in Elution Buffer. The purified plasmid DNA can be used in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing. The kit utilizes an advanced silica-based membrane technology in the form of a convenient spin column, which helps to recover upto 20 g of high- or low-copy plasmid DNA from 1-5 ml of E. coli culture per isolation procedure.