HiPurA Soil DNA Purification Kit-MB542-20PR

HiPurA Soil DNA Purification Kit-MB542-20PR

₹ 5,025.00 *

Brand: HIMEDIA

Catalog No.: MB542-20PR

Quantity/Unit: 20pr*/EA

Usually Shipped in: 2-3 Weeks

Expiry Date : 01-Jan-1970

Qty:
Min Orderable Qty : 1 Pack

Free Shipping on orders over USD10000

For lab/research use only, unless otherwise specified

*Shipping charges extra. You may have to bear duty and taxes extra as per your country.

Product Description

HiPurA Soil DNA Purification Kit is supplied by HiMedia. HiPurA Soil DNA Purification Kit provides a fast and easy method for purification of total DNA from environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, manure and other common soil types, for reliable applications in PCR and other downstream applications. The HiPurA DNA purification system combines the reversible nucleic acid binding properties of HiElute Miniprep Spin Column (Capped) with an efficient buffer system, which eliminates PCR inhibiting compounds such as humic acid from soil samples. This kit contains:

Inhibitor Removal Solution (IRSH)

Soil Lysis Solution (SL)

Binding Solution (SB)

Wash Solution Concentrate (WSP)

Elution Buffer (ET) [10mM Tris-Cl, pH 8.5]

HiBead Tubes

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

HiPurA Soil DNA Purification Kit simplifies isolation of DNA from soil using spin column technique. The kit is optimized to process 250-500 mg of soil sample. Soil samples possess typically many compounds that can inhibit downstream enzymatic reactions. The HiPurA Soil DNA Purification Kit effectively removes these inhibitory compounds during the extraction process. The Inhibitor Removal Solution (IRSH), provided in the kit, is effective in removing PCR inhibitors from even the most difficult soil types. The soil sample is lysed and homogenized in a bead-beating step. The total genomic DNA obtained is bound to silica membrane in a spin column format, washed and eluted from the membrane. The purified DNA can directly be used for PCR analysis and other downstream applications.