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HiPurA Sperm Genomic DNA Purification Kit-MB522-50PR

HiPurA Sperm Genomic DNA Purification Kit-MB522-50PR

₹ 9,785.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MB522-50PR
  • Quantity/Unit: 50pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HiPurA Sperm Genomic DNA Purification Kit is supplied by HiMedia. HiPurA Sperm Genomic DNA Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR and Southern blotting technique. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality genomic DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction endonuclease digestion, PCR and Southern blotting. This kit contains:

Resuspension Solution (1X PBS)

Semen Wash Buffer Concentrate (SEW)

Sperm Lysis Buffer (SL)

Lysis Solution (C1)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

DL-Dithiothreitol (DTT)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from sperm samples with spin column procedure. Following lysis, DNA binds to the silica gel membrane of the HiElute Miniprep Spin Column (Capped) to yield high purity DNA. Two rapid wash steps remove trace salts and protein contaminants resulting in the elution of high quality DNA in the Elution Buffer (ET) provided with the kit.

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