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HiPurA Stool DNA Purification Kit-MB544-20PR

HiPurA Stool DNA Purification Kit-MB544-20PR

₹ 5,175.00 *
  • Brand: HIMEDIA
  • Catalog No.: MB544-20PR
  • Quantity/Unit: 20pr*/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


HiPurA Stool DNA Purification Kit is supplied by HiMedia. HiPurA Stool DNA Purification Kit provides a fast and easy method for purification of stool DNA for reliable applications in PCR and Southern blotting technique, etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality DNA is obtained from various species. The purified DNA obtained is compatible with downstream applications such as restriction enzyme digestion, hybridization techniques, PCR and Southern blotting. This kit contains:

TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)

Lysis Solution (AL)

Stool Lysis Buffer (SL1)

Inhibitor Removal Solution (IRSH)

Wash Solution Concentrate (WSP)

Binding Solution (SB)

Elution Buffer (ET) [10mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This HiPurA Stool DNA Purification Kit simplifies isolation of DNA from stool using spin column technique. The procedure is optimized for rapid and reliable isolation of high-quality total DNA from upto 250 mg of fresh or frozen stool samples. Stool samples possess typically many compounds that can degrade DNA and inhibit downstream enzymatic procedures. The HiPurA Stool DNA Purification Kit contains a unique solution, Inhibitor Removal Solution (IRSH) (DS0066) to remove these inhibitory compounds at an early stage during the extraction process. The total genomic DNA obtained is bound to silica membrane in a spin column format, washed and eluted from the membrane. The purified DNA can directly be used for PCR analysis and other downstream applications.

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