HiPurA Yeast Plasmid DNA Purification Kit is supplied by HiMedia. HiPurA Yeast Plasmid DNA Purification Kit provides a fast and easy method for purification of high quality plasmid DNA from yeast cultures. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure plasmid DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality DNA is obtained. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing. This kit contains:

Resuspension Solution (HP1)

Lysis Solution (HP2)

Neutralization Solution (HN3)

Wash Solution (HPB)

Wash Solution Concentrate (HPE)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of plasmid DNA from yeast by the spin-column procedure. In the procedure, the cell wall of yeast cells is lysed enzymatically with lyticase/zymolyase in presence of a buffer. Spheroplasts are then collected by centrifugation and subjected to alkaline lysis, after which it is applied on to the silica column for binding of the DNA molecules in the presence of high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in Elution Buffer. The plasmid yields depend on copy number, yeast strain and growth conditions. The purified plasmid DNA can be used in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing.