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HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)

HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)

₹ 22,450.00 *
(0)
  • Brand: HIMEDIA
  • Catalog No.: MBPP005-5NO
  • Quantity/Unit: 5no/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) is supplied by HiMedia. It is a fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. The chelating group of Ni-TED is based on TED (tris-carboxymethyl ethylene diamine), a strong pentadentate metal chelator. TED occupies five of the six binding sites in the coordination sphere of the Ni2+ ion, the remaining coordination site of Ni2+ is available for protein binding. Compared to TED, other chelating groups such as NTA (nitrilotriacetic acid) have four binding sites available for the Ni2+ ion, the remaining two sites of Ni2+ are available for protein binding.