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Hugh Leifson Medium

Hugh Leifson Medium

₹ 1,146.00 *
(0)
  • Brand: Micromaster
  • Catalog No.: DM370-100g
  • Quantity/Unit: 100g/Pack
  • Usually Shipped in: 1-2 Weeks

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Min Orderable Qty : 1 Pack


For lab/research use only, unless otherwise specified

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High grade Hugh Leifson Medium is supplied by Micromaster. It is available in the form of a light yellow to bluish green homogenous free flowing powder. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

  • Quantity provided: 100 g/pack

Hugh Leifson Medium is used to distinguish between anaerobic and aerobic breakdown of carbohydrate (glucose). Hugh Leifson Medium was formulated by Hugh and Leifson . They described the taxonomic significance of fermentative and oxidative metabolism of carbohydrates in gram-negative intestinal bacteria. It is recommended by BIS for the isolation and cultivation of Vibrio cholerae and other Vibrio species which cause food poisoning. The medium contains a high concentration of carbohydrate and low concentration of peptone to avoid the possibility of an aerobic organism utilizing peptone and producing an alkaline condition which would neutralize slight acidity produced by an oxidative organism. Dipotassium phosphate promotes fermentation and acts as pH controlling buffer. Agar concentration enables the determination of motility and aids in distribution of acid throughout the tube produced at the surface of medium. Oxidative organisms produce acid in unsealed tube with little or no growth and no acid formation in sealed tube while fermentative organisms produce acid in both sealed and unsealed tubes.

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