IMDM w/ L-Glutamine and 25mM HEPES Buffer w/o Glucose and Sodium bicarbonate

IMDM w/ L-Glutamine and 25mM HEPES Buffer w/o Glucose and Sodium bicarbonate

₹ 6,100.00 *

Brand: HIMEDIA

Catalog No.: AT230-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine and 25mM HEPES Buffer Without Glucose and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.2 -5.8 whereas the pH with Sodium Bicarbonate is between 6.7 -7.3. The osmolality without Sodium Bicarbonate is in the range of 210.00 -250.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (219.00)

• Magnesium sulphate anhydrous (97.72) 

• Potassium chloride (330.00) 

• Potassium nitrate (0.076)

• Sodium chloride (4505.00)

• Sodium dihydrogen phosphate, anhydrous (109.00)

• Sodium selenite (0.017)

• Amino acids

• Choline chloride (4.00)

• D-Biotin (0.013) 

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00) 

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.40) 

• Thiamine hydrochloride (4.00) 

• Vitamin B12 (0.013) 

• i-Inositol (7.20) 

• HEPES Buffer (5958.00)

• Sodium pyruvate (110.00)

Iscove's Modified Dulbecco's Medium is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B-lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. This product contains L-glutamine and 25mM HEPES buffer but does not contain phenol red. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

 

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