Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine Without HEPES buffer and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.6 -6.2 whereas the pH with Sodium Bicarbonate is between 7.6 -8.2. The osmolality without Sodium Bicarbonate is in the range of 190.00 -230.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 260.00 -300.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (219.00)

Disodium hydrogen phosphate anhydrous (109.00)

Magnesium sulphate anhydrous (97.73)

Potassium chloride (330.00)

Potassium nitrate (0.076)

Sodium chloride (4505.00)

Sodium selenite (0.017)

Amino acids

Choline chloride (4.00)

D-Biotin (0.013)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.40)

Thiamine hydrochloride (4.00)

Vitamin B12 (0.013)

i-Inositol (7.20)

D-Glucose (4500.00)

Phenol red sodium salt (15.00)

Sodium pyruvate (110.00)

Iscove's Modified Dulbecco's Medium is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. This product contains L-glutamine but does not contain HEPES buffer. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.