Catalog No.: 210210105
CAS No: 367-93-1
Quantity/Unit: 5 g/Bottle
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- PRODUCT DETAILS
Isopropyl-β-D-thiogalactopyranoside by MP Biomedicals is a high grade reagent also known as IPTG. This molecular compound imitates allolactose, a lactose metabolite which stimulates transcription in the lac operon. This compound can hence induce protein expression by activating genes under the control of the lac operator, such as of the enzyme, beta-galactosidase. IPTG, however cannot be hydrolysed by beta-galactosidase. It effectively induces protein expression in the concentration range of 100 μM to 3.0 mM. This reagent is easy to prepare by just dissolving the powder in water. • CAS No.: 367-93-1 • Quantity Provided: 5 gm per bottle • Molecular Formula: C9H18O5S • Molecular Weight: 238.3 • Soluble in water and methanol (50 mg/mL give a clear solution) IPTG is commonly used in cloning procedures that require the induction of β-galactosidase activity. This reagent is used together with X-gal in the blue-white screen test. Blue-white screen allows colonies which have been transformed with the recombinant plasmid rather than a non-recombinant one to be identified in cloning experiments. IPTG functions by binding to the lacI repressor after which it alter its conformation, which results in the activation of the β-galactosidase coding gene lacZ without restriction. These reagents are essentials in molecular biology labs and can be used in various gene expression studies.