Levine Eosin-Methylene Blue Agar (EMB Agar ,Levine)
₹ 1,033.00 *
Catalog No.: DM094I-100g
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High grade EMB (eosin methylene blue) agar, Levine is supplied by Micromaster. It is available in the form of a light pink to purple homogeneous free flowing powder which has a peptone-like odour. It has a pH in the range of 6.9 to 7.3. It contains a blend of two stains, eosin and methylene blue in the ratio of 6:1. For maximum efficiency, it must be stored below 30°C in tightly closed container. This is in compliance with IP.
• Quantity provided: 100 g/pack
EMB Agar, Levine is highly recommended for the isolation, enumeration and differentiation of members of Enterobacteriaceae from clinical and non-clinical samples. It is a preferred choice for detecting and differentiating between the members of the coliform group. EMB Agar, Levine is also used as a differential microbiological medium, which slightly inhibits the growth of gram-positive bacteria and provides a colour indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella). The presence of Eosin-Y and methylene blue makes the medium slightly selective and inhibits certain gram-positive bacteria. These dyes are used to differentiate between lactose fermenters and non-fermenters. Vigorous fermenters of lactose or sucrose will produce quantities of acid sufficient to form the dark purple dye complex whereas non-fermenters produce colourless colonies. Peptone acts as source of carbon, nitrogen, and other essential growth nutrients. Eosin-Y and methylene blue serve as differential indicators. Phosphate is used as a buffer for the medium.