Luria Bertani Agar, Miller (Miller Luria Bertani Agar) is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 7.3 to 7.7. It is advisable to store this product below 30C.

Quantity provided: 500 g/pack

Luria Bertani Agar Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms. Luria Bertani Agar is prepared as described by Lennox for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.