High grade Lysine Decarboxylase Broth is supplied by Micromaster. It is available in the form of a light yellow to greenish yellow homogeneous free flowing powder with a pH in the range of 6.6 to 7.0. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 100g /pack

Lysine Decarboxylase Broth is used for differentiating Salmonella arizonae from the Bethesda Ballerup group of Enterobacteriaceae. Decarboxylase media was described by Moeller, for detecting lysine and ornithine decarboxylase and arginine dihydrolase. Falkow developed a lysine decarboxylase medium for the identification and differentiation of Salmonella and Shigella. Lysine Decarboxylase Broth is especially suited to study the decarboxylase reactions for members of Enterobacteriaceae other than Klebsiella and Enterobacter. Gelatin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate. Bromocresol purple is the pH indicator. Lysine is added to detect the production of the specific enzyme.

During the initial stages of incubation, fermentation of dextrose by the organisms leads to acid production, which causes the bromocresol purple indicator to turn yellow. The acidic condition thus generated stimulates decarboxylase activity, which leads to decarboxylation of lysine to cadavarine. The alkaline conditions generated due to cadaverine production cause the bromocresol purple indicator to revert to purple colour. If the organisms do not produce decarboxylase enzyme, the colour of the medium remains yellow.