High grade M-Tergitol 7 Agar Base is supplied by Micromaster. It is available in the form of a yellow to blue coloured homogeneous free flowing powder with a pH in the range of 7.2 to 7.6. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 500g /pack

McFeters, Cameron and LeChevallier modified Tergitol 7 Agar to improve its selective and differential properties for the recovery of stressed coliforms from chlorinated water. They have reported that the selective media such as M-Endo Agars used to isolate gram-negative bacteria recovered only 30% or less as compared to recovery between 71 & 100% of injured coliforms on Tergitol 7 Agar. In their study of water samples, including samples containing laboratory-stressed coliforms and surface and drinking water samples, M-Tergitol 7 Agar Base recovered 43% more coliforms than on M-Endo Agar and 36% more coliforms than by using M-Endo Agar with a resuscitation technique. The casein enzymic hydrolysate and peptic digest of animal tissue provide necessary nitrogenous growth factors. Yeast extract is the source of B-vitamins and organic nitrogen and carbon compounds. Lactose is the fermentable carbohydrate. Microorganism fermenting lactose produces yellow colonies due to reaction with bromothymol blue and bromocresol purple indicators. These indicators also act as inhibitors of non-coliform microbes. Sodium heptadecyl sulphate (Tergitol 7) and polyoxyethylene ether W-1 are surface active agents which inhibit growth of gram-positive bacteria as well as swarming of Proteus . Inhibition of gram-positive bacteria can be improved by aseptically adding penicillin G (1.0 g/ml) after autoclaving and cooling to 45C.