DNA methylation occurs mainly at the 5-position of cytosine rings (5-methylcytosine (5mC)). The 5mC bases are found largely in genomic regions with a high frequency of cytosines and guanines, called CpG islands. Methyl-CpG binding protein 2 (MeCP2) specifically binds to methylated promoters on CpG islands and mediates gene silencing by recruiting corepressor complexes.1 The Methyl-CpG Binding Domain (MBD) protein family consists of five members: MeCP2, MBD1, MBD2, MBD3 and MBD4.2,3,4 The sequence similarity between the MBD proteins is limited to the MBD domains.5 In vitro work suggests high affinity binding of MeCP2 is facilitated by DNA fragments containing A/T bases ([A/T]4) adjacent to the methyl-CpG.6 Mutations in the MeCP2 gene have been linked to the X-linked neurological disorder, Rett Syndrome.7Technical InformationSynonymsMethyl-CpG Binding Protein 2Purity95%Sourcerecombinant N-terminal GST-tagged protein expressed in E. coliMW37 kDaFormulation50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM -mercaptoethanolUniProt Accession P51608