MEM (Alpha Modification) w/o L-Glutamine, Deoxyribonucleosides, Ribonucleosides, NaHCO3
₹ 1,855.00 *
Catalog No.: AT081A-5L
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Minimum Essential Medium Eagle (MEM) (Alpha Modification) Without L-Glutamine, Deoxyribonucleosides, Ribonucloesides and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.3 -4.9 whereas the pH with Sodium Bicarbonate is between 7.1 -7.7. The osmolality without Sodium Bicarbonate is in the range of 250.00 -290.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 295.00 -335.00 Osm/Kg H20. It is advisable to store this product between 2-8C. The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
Media composition (in mg/L) is as follows:
Calcium chloride dihydrate (265.00)
Magnesium sulphate anhydrous (97.72)
Potassium chloride (400.00)
Sodium chloride (6800.00)
Sodium dihydrogen phosphate anhydrous (122.00)
Choline chloride (1.00)
Folic acid (1.00)
L-Ascorbic acid (50.00)
Pyridoxal hydrochloride (1.00)
Thiamine hydrochloride (1.00)
Vitamin B12 (1.36)
Lipoic acid (0.20)
Phenol red sodium salt (11.00)
Sodium pyruvate (110.00)
Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. This product is the alpha modification of Minimum Essential Medium Eagle without non-essential amino acids, L-glutamine, deoxyribonucleosides and ribonucleosides. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.