MEM w/ Earles salts, L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate

MEM w/ Earles salts, L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate

₹ 6,100.00 *

Brand: HIMEDIA

Catalog No.: AT074-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Minimum Essential Medium Eagle (MEM) With Earle's salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of a white to light pink, homogenous powder. The pH without Sodium Bicarbonate is between 5.2 -5.8 whereas the pH with Sodium Bicarbonate is between 6.6 -7.2. The osmolality without Sodium Bicarbonate is in the range of 280.00 -320.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows: 

• Calcium chloride dihydrate (265.00) 

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00) 

• Sodium chloride (6800.00)

• Sodium dihydrogen phosphate anhydrous (122.00)

• Amino acids

• Choline chloride (1.00)

• D-Ca-Pantothenate (1.00)

• Folic acid (1.00)

• Nicotinamide (1.00)

• Pyridoxal hydrochloride (1.00)

• Riboflavin (0.10)

• Thiamine hydrochloride (1.00)

• i-Inositol (2.00)

• D-Glucose (1000.00)

• HEPES buffer (5958.00) 

• Phenol red sodium salt (11.00) 

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. HEPES is a zwitterionic buffer having a pKa of 7.3 at 37ºC, which prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

 

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