MEM w/ Earles salts, NEAA, Sodium bicarbonate and L-Glutamine
₹ 2,215.00 *
Catalog No.: AL047H-500ML
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Minimum Essential Medium (MEM) is supplied by HiMedia. It is available in the form of an orangish red coloured clear solution which has a pH in the range of 7.0 to 7.6 and an osmolality between 295.00-335.00 Osm/Kg H20. MEM contains Earle's salts, L-glutamine, sodium bicarbonate and non-essential amino acids. It is advisable to store this product between 2-8⁰C, away from bright light. The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures. This medium is also hybridoma tested.
• Quantity provided: 500ml /EA
Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.