MEM w/ Earles Salts w/o L-Glutamine, NEAA, Phenol red and Sodium bicarbonate

MEM w/ Earles Salts w/o L-Glutamine, NEAA, Phenol red and Sodium bicarbonate

₹ 3,250.00 *


Catalog No.: AT159A-10X1L

Quantity/Unit: 10x1lt/EA

Usually Shipped in: 2-3 Weeks

Min Orderable Qty : 1 Pack

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For lab/research use only, unless otherwise specified

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Product Description

Minimum Essential Medium Eagle (MEM) With Earle's salts Without L-Glutamine, NEAA, Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.9 -6.5 whereas the pH with Sodium Bicarbonate is between 7.3 -7.9. The osmolality without Sodium Bicarbonate is in the range of 220.00 -260.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 260.00 -300.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00) 

• Sodium chloride (6800.00) 

• Sodium dihydrogen phosphate anhydrous (122.00)

• Amino Acids

• Choline chloride (1.00)

• D-Ca-Pantothenate (1.00) 

• Folic acid (1.00) 

• Nicotinamide (1.00)

• Pyridoxal hydrochloride (1.00)

• Riboflavin (0.10)

• Thiamine hydrochloride (1.00) 

• i-Inositol (2.00)

• D-Glucose (1000.00)

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. This product does not contain non-essential amino acids, phenol red and L-glutamine. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.


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