MEM w/ Hanks' salts, L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate
₹ 6,100.00 *
Catalog No.: AT075-20L
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Minimum Essential Medium Eagle (MEM) With Hank's salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.5 -6.1 whereas the pH with Sodium Bicarbonate is between 6.1 -6.7. The osmolality without Sodium Bicarbonate is in the range of 295.00 -335.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 305.00 -345.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
• Quantity provided: 20lt /EA
Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. This product contains Hank's salts, L-glutamine and 25mM HEPES buffer. HEPES is a zwitterionic buffer having a pKa of 7.3 at 37ºC, which prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.