High grade Milk Agar w/ Cetrimide (Twin Pack) is supplied by Micromaster. It is available as two parts, part A is a white to cream homogenous free flowing powder and part B is a cream to yellow homogenous free flowing powder. It has a pH in the range of 7.1 to 7.5. It is advisable to store this product below 30C.

• Quantity provided: 500 g/pack

Milk Agar was modified by Brown and Scott, by adding cetrimide for the confirmation of Pseudomonas aeruginosa in swimming pool water. Swimming pool water is generally chlorinated potable water but it can also be from thermal springs or salt water. Microorganisms of concern are typically those from the body of the bathers, including the orifices. Milk Agar with Cetrimide is formulated in accordance with ISO Committee under the specifications ISO 8360-1: 1988 for the detection and enumeration of P.aeruginosa from water. Strains of P.aeruginosa are identified by their pigment production i.e. pyocyanin since P.aeruginosa is the only species of Pseudomonas or gram-negative rod known to excrete pyocyanin. P.aeruginosa hydrolyzes casein and produces a yellowish to green diffusible pigment on Milk Agar. Milk, skim milk powder, peptic digest of animal tissue and yeast extract provide all the necessary nutrients mainly nitrogenous for the multiplication of P.aeruginosa. P.aeruginosa forms yellowish green colonies on this medium. Cetrimide acts as a quaternary ammonium, cationic detergent that causes release of nitrogen and phosphorus from bacterial cells other than P.aeruginosa.