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Mitis Salivarius Agar

Mitis Salivarius Agar

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  • Brand: Micromaster
  • Catalog No.: DM168-500g
  • Quantity/Unit: 500g/Pack

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High grade Mitis Salivarius Agar Base is supplied by Micromaster. It is available in the form of a light yellow to light blue homogeneous free flowing powder with a pH in the range of 6.8 to 7.2. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 500g /pack

Mitis Salivarius Agar is recommended for the isolation of Streptococci, especially Streptococcus mitis, Streptococcus salivarius and Enterococcus faecalis from grossly contaminated specimens. Streptococcus species are mostly commensal residents of the mouth and throat, though several may act as opportunistic pathogens and a few as primary pathogens. Streptococcus viridans group consists of Streptococcus salivarius and Streptococcus mitis. Mitis Salivarius Agar is formulated as per Chapman. This medium (with 1% potassium tellurite) is a highly selective medium, which enables to isolate streptococci from highly contaminated specimens like exudates from body cavities and faeces etc., as it inhibits a wide variety of bacteria. Casein enzymic hydrolysate and peptic digest of animal tissue in the medium provide the essential growth nutrients. Dextrose and sucrose are the fermentable carbohydrates. Dipotassium phosphate buffers the medium. Trypan blue is an acidic, blue diazo dye while crystal violet is a basic dye and also a bacteriostatic agent, which inhibits many gram-positive organisms. Potassium tellurite also helps to make the medium selective for streptococci. Occasionally Streptococcus mutans strains may be inhibited on Mitis Salivarius Agar Base due to the high concentration of trypan blue in the medium.

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