Murashige & Skoog Plant Salt Mixture without NH4NO3, KNO3 and CaCl2, is supplied by HiMedia. It is available in the form of a white to off-white, homogenous, free flowing powder with a pH in the range of 3.5 to 4.5. Since this product is extremely hygroscopic, it is advisable to store it at 2-8C, in air tight containers. The composition (mg/ml) is as follows:

Potassium nitrate (950.00)

Ammonium nitrate (825.00)

Calcium chloride.2H2O (220.00)

Manganese sulphate.H2O (16.90)

Boric acid (6.20)

Potassium iodide (0.83)

Molybdic acid (sodium salt).2H2O (0.25)

Zinc sulphate.7H2O (8.60)

Copper sulphate.5H2O (0.025)

Cobalt chloride.6H2O (0.025)

Ferrous sulphate.7H2O (27.80)

EDTA disodium salt.2H2O (37.30)

Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. It is commonly used for micro propagation, organ culture, callus culture and suspension culture. Murashige & Skoog plant salt mixture has been specially formulated for plant cell, tissue and organ cultures. The mixture contains macroelements, microelements and iron source.