Murashige & Skoog Shoot Multiplication Medium B Macroelements, is supplied by HiMedia. It is available in the form of a white to off-white, homogenous, free flowing powder with a pH in the range of 4.2 to 5.2. Since this product is extremely hygroscopic, it is advisable to store it at 2-8C, in air tight containers. The composition (mg/ml) is as follows:

Potassium nitrate (1900.00)

Ammonium nitrate (1650.00)

Calcium chloride.2H2O (440.00)

Magnesium sulphate (180.69)

Potassium phosphate monobasic (170.00)

Sodium phosphate monobasic (147.81)

Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. It is commonly used for micro propagation, organ culture, callus culture and suspension culture. Murashige & Skoog shoot multiplication medium B macroelements has been specially formulated for plant cell, tissue and organ cultures. Potassium nitrate and ammonium nitrate serves as the sources of nitrate. Potassium phosphate monobasic and sodium phosphate monobasic serves as the sources of phosphate.