Murashige & Skoog Vitamins (100X) is supplied by HiMedia. It is available in the form of a white to off-white, homogenous, free flowing powder with a pH in the range of 3.3 to 4.3. Since this product is extremely hygroscopic, it is advisable to store it at 2-8C, in air tight containers. The composition (mg/ml) is as follows:
myo - Inositol (100.00)
Thiamine hydrochloride (0.10)
Pyridoxine hydrochloride (0.50)
Nicotinic acid (Free acid) (0.50)
Glycine (Free base) (2.00)
Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. It is commonly used for micro propagation, organ culture, callus culture and suspension culture. Murashige & Skoog vitamins (100X) powder is ready to use mixture of vitamins and amino acid which is sufficient to prepare 100 litres of medium from content of one vial. The vial contains 10.31 gm of powdered vitamin mixture.