MYP Agar Base (Phenol Red Egg Yolk Polymyxin Agar Base) is supplied by Micromaster. It is available in the form of a light yellow to light pink homogeneous free flowing powder which has a pH in the range of 7.1 to 7.3. It is advisable to store this product below 30C in a tightly closed container.

Quantity provided: 100 g/pack

Mannitol Yolk Polymyxin (MYP) Agar was formulated by Mossel et al and is recommended by APHA for enumeration of Bacillus cereus. When present in large numbers in certain foodstuffs, Bacillus cereus can produce metabolites responsible for the clinical symptoms of food poisoning. MYP Agar Base is recommended by BIS for isolation and enumeration of Bacillus cereus. The medium contains peptic digest of animal tissue and meat extract which provide nitrogen source. Mannitol fermentation can be detected with the phenol red, which yields yellow colour to the mannitol fermenting colonies. Added egg yolk emulsion helps in differentiation of lecithinase producing colonies which are surrounded by a zone of white precipitate. Addition of Polymyxin B Sulphate helps to restrict growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. These differentiating media allow differentiation of Bacillus cereus from other Bacillus species by its inability to ferment mannitol and poor sporulation. Acid produced by organisms other than Bacillus cereus often diffuse through the medium, making it difficult to distinguish between mannitol fermenters and nonfermenters.