Nutrient Broth is supplied by HiMedia. It is available in the form of a cream to yellow homogeneous free flowing powder with a pH in the range of 7.2 to 7.6. Nutrient Broth is used for the general cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids. It is advisable to store this product between 10-30C. In order to prevent lump formation due to the hygroscopic nature of the product, it should be properly stored dry, after tightly capping the bottle, after opening. Composition (in gm/L) is as follows:

Peptone (5.00)

Sodium chloride (5.00)

HM peptone (1.50)

Yeast extract (1.50)

Nutrient media is a basic culture media, which is used for maintaining microorganisms; cultivating fastidious organisms by enriching with serum or blood . It is also used for purity checking prior to biochemical or serological testing. Nutrient Broth is based on the formula originally designed for use in the Standard Method for Examination of Water and Waste water. It is one of the several non-selective media useful in routine cultivation of microorganisms. It is suitable for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms. Peptone, HM peptone B and yeast extract provide nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.