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Nutrient Mixture F-12 Ham, Coons Modificationw/ L-Glutamine w/o Sodium bicarbonate-AT146-1L

Nutrient Mixture F-12 Ham, Coons Modificationw/ L-Glutamine w/o Sodium bicarbonate-AT146-1L

₹ 825.00 *
  • Brand: HIMEDIA
  • Catalog No.: AT146-1L
  • Quantity/Unit: 1lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


Nutrient Mixture F-12 Ham, Coon's Modification With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.8 -5.4 whereas the pH with Sodium Bicarbonate is between 7.3 -7.9. The osmolality without Sodium Bicarbonate is in the range of 270.00 -310.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 330.00 -370.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (165.00)

Copper sulphate pentahydrate (0.0025)

Disodium hydrogen phosphate anhydrous (132.04)

Ferric sulphate heptahydrate (0.83)

Magnesium chloride hexahydrate (106.00)

Magnesium sulphate anhydrous (50.80)

Potassium chloride (305.00)

Potassium dihydrogen phosphate (68.00)

Sodium chloride (7530.00)

Zinc sulphate heptahydrate (0.14)

Amino acids

Biotin (0.0073)

Choline chloride (13.96)

D-Ca-Pantothenate (0.48)

Folic acid (1.32)

L-Ascorbic acid (45.00)

Nicotinamide (0.04)

Pyridoxine hydrochloride (0.06)

Riboflavin (0.04)

Thiamine hydrochloride (0.34)

Vitamin B12 (1.36)

i-Inositol (18.02)

D-Glucose (2000.00)

Hypoxanthine sodium salt (4.04)

Linoleic acid (0.09)

Lipoic acid (0.21)

Phenol red sodium salt (1.24)

Putrescine dihydrochloride (0.30)

Sodium pyruvate (220.00)

Thymidine (0.70)

Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F12, Coon's modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with Sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid.

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