PAREEKSHAK HIV 4th Gen. Ag & Ab COMBO ELISA KIT is supplied by BHAT BIO-TECH.It is an In vitro Antigen and Antibody sandwich elisa where in the HIV antigen p24 and Isotypes of HIV antibodies are detected simultaneously along with antibodies to HIV-2.This method enables the early detection of HIV reducing the window period of detection.It is an immunoassay which employs r-proteins of HIV-1 gp41, C-terminus of gp120, HIV-2 recombinant protein of gp36 along with Monoclonal Anti p24 antibodies to HIV-1 for the detection of antigen & antibodies to HIV 1&2 in human serum or plasma. The use of r-proteins offers the advantage of high degree of specificity and sensitivity due to multiple epitopes.It utilizes a unique combination of HIV-1 & 2 antigens of the virus to selectively detect all subtypes of HIV-1 & 2 Virus in human serum/plasma with a high degree of sensitivity and specificity. The kit must be stored between 2-8 C. The bag containing micro titer plate must be brought to Room temperature (20-30C) before opening, to avoid condensation in the wells. Unused wells should be sealed in the bag, and refrigerated (2-8 C). After opening the sealed pouch, unused strips are stable for 3 months at 2-8 C in the original pack sealed with tape.

Contents of Kit:

Anti-P24 & HIV Rec Antigen coated microwells (Ready to use)

Wash solution (concentrated 10x)

Biotinated Anti-P24 Antibody Conjugate (50x Concentrated)

Enzyme Conjugate Dilluent

Streptavidin-HIV-1/2 HRP Conjugate (100x Concentrated)

TMB Substrate

TMB Diluent

Stop Solution (Ready to use)

Positive Control (Ready to use)

Negative Control (Ready to use)

Adhesive Slips

Pack Insert

Features:

4th generation assay.

PAREEKSHAK HIV-4th GEN. Ag & Ab COMBO ELISA TEST is an immunoassay which employs r-proteins of HIV-1 gp41, C-terminus of gp120, HIV-2 recombinant protein of gp36 along with Monoclonal Anti p24 antibodies to HIV-1 for the detection of antigen & antibodies to HIV 1&2 in human serum.

Breakaway micro well strips.

It is an indirect antibody EIA assay.

Short assay procedure: 120 minutes

Three incubations.

Longer shelf life of 18 months at 2-80C.

Sensitivity above 99.5% & specificity above 99%.